Ecocyte Bioscience offers In Vitro Transcription (IVT) services to suit your needs! Whether you are starting from the procurement of the cDNA or using cDNA your lab has at hand, we will be able to help you through the process of transcription so you may have the most optimal results in your research.
Following restriction digestion as well as spin column clean-up of the now linearized vector containing your gene of interest with the right enzyme, the first quality control step ensures suitability for downstream application. The IVT is then followed by a further spin column clean-up and quality control step, with the results being shared with the you as PDF files.
Upon request, functionality of your transcribed genes of interest can be tested directly in Two-Electrode Voltage-Clamp (TEVC) or swelling assays on Xenopus laevis oocytes.
IVT services offered by us utilize bacteriophage-derived T7 RNA polymerase, with addition of 5’ m7G-cap and 3’ polyA tails for increased stability and downstream translation. More-so, the addition of a Kozak sequence is generally done to even further improve translation. Ancillary translation sequences expressed by the target cells for in vitro translation can also be added upon request as well. Post-transcriptional DNase treatment removes any DNA template.
RNA that is synthesized through this process can be used in a wide range of applications, ranging from in vitro translation, biochemical studies and protein expression to electrophysiology.
since 2006 in Europe
since 2010 in US
2,5+ Mil Xenopus Oocytes in Europe
1,3+ Mil Xenopus Oocytes in US and Canada
300+ customers in Europe
500+ customers in US and Canada
Hundreds of peer reviewed scientific publications by using our products.
Our service is:
- Time effective: Fast and reliable
- Quality effective: Continuous quality – due to optimized procedures.
- Cost effective: calculate your research costs accurately based on required services.
- Work effective: streamline your workload
- Space effective: reduction of required lab space
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It is with this new service that we hope to simplify your research needs to another degree, and for labs across the US to spend more time on the projects that matter. If you would like any further information or an estimate for any of our services, please use the contact form above. We look forward to working with you!
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