NFR Product information
Normal Frog Ringer (NFR) is used for lab experiments with Xenopus laevis oocytes to guarantee a physiological environment with respect to homeostasis, pH and osmolarity.
- 500 ml Ready to Use NFR solution or 10x concentrate available
- autoclaved
Instructions for use:
Ready to use:
Direct use
10x concentrate:
For 1000 ml NFR Solution:
- Pour 100 ml NFR (10x) into a flask
- Add deionized water to a final volume of 1000 ml
Adjust to pH 7.4
Use immediately once opened. Store at 4 – 8 °C
Ional composition of NFR Solution
NaCl = 90 mM
KCl = 2 mM
CaCl2 = 2 mM
MgCl2 = 1 mM
HEPES = 5 mM