NFR Product information

Normal Frog Ringer (NFR) is used for lab experiments with Xenopus laevis oocytes to guarantee a physiological environment with respect to homeostasis, pH and osmolarity.

  • 500 ml Ready to Use NFR solution or 10x concentrate available
  • autoclaved

Instructions for use:

Ready to use:
Direct use

10x concentrate:
For 1000 ml NFR Solution:

  1. Pour 100 ml NFR (10x) into a flask
  2. Add deionized water to a final volume of 1000 ml

Adjust to pH 7.4

Use immediately once opened. Store at 4 – 8 °C

Ional composition of NFR Solution

NaCl = 90 mM
KCl = 2 mM
CaCl2 = 2 mM
MgCl2 = 1 mM
HEPES = 5 mM

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